Montpellier platform

Institute for Regenerative Medicine and Biotherapies (IRMB )

Université de Montpellier, Inserm U1183




eXPERTISE

The ECELLFRANCE Montpellier platform conducts research on the immunomodulatory and regenerative properties of adult mesenchymal stem cells (MSC) and on their therapeutic use in autoimmune and osteo-articular pathologies.

 

SERVICE DELIVERIES OFFERED

  • In vitro cellular biology
    • Human and murine MSC culture from different tissue source
    • MSC functional evaluation: ability to differentiate into chondrocytes, osteoblasts and adipocytes, immunomodulatory properties
    • Differentiation  models for human and murine dendritic cells
    • Cell isolation (NK, T and B lymphocytes) /  cell activation
  • In vivo experimental models 
    • Several experimental models and clinical and therapeutical follow-up after treatment by cellular therapy/biothrapy/therapeutic antibody/drug
    • Murine models: collagen-induced arthritis (CIA), arthritis by serum transfer (K/BxN), collagen-induced osteoarthritis (CIOA),  menisco-tibial ligament destabilization (MMD), HOCl-induced systemic scleroderma, delayed type hypersensitivity (DTH), colitis (SCID mice)
    • Immune response evaluation
    • MSC biodistribution and toxicity studies 
    • Imaging and histomorphometric analysis of  bone damages by X ray microtomography
    • Imaging and histomorphometric analysis of  cartilage damages by confocal microscopy
    • Clinical response's semi-quantitative analyses by histological scoring 
Mouse tibial tray by confocal microscopy
Mouse tibial tray by confocal microscopy
Mouse knee by microCT
Mouse knee by microCT
Safranine-O-fast-green colored mouse knee
Safranine-O-fast-green colored mouse knee

  • Clinical reserach
    • Immunomonitoring of immune cell sub-population: percent, numeration, differentiation and activation status quantitative analyses of monocyte, dendritic cell and T, B and NK lymphocyte sub-populations 
    • Technical help to set up flow cytometry multiparametric panels 
                                                                                                                                                                                               Immunomonitoring graphical abstract
Immunomonitoring graphical abstract

EQUIPEMENT

  • X ray microtomography, SkyScan 1176 model
  • Confocal laser scanning microscopy, Leica Sp5 model
  • Fortessa cytometer, LSRII from Beckton Dickinson
  • Automax from Miltenyi

Keywords

  • Mesenchymal stem/stromal cells (MSC)
  • Differentiation
  • Immunomonitoring
  • Osteo-articular and auto-immune diseases
  • Cell therapies
  • Biotherapies
université Montpellier